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Count and Measure Solution module

Olympus has released its Count and Measure Solution module for the cellSens Dimension software suite. The module makes it simple to design and automate image analysis protocols by providing advanced object detection and structure differentiation. This maximises accuracy and reproducibility while minimising the time users must devote to manual image processing.

The system can be configured easily to recognise objects and structures based on user specifications by employing a range of threshold-based methods, thereby defining rules with which to process a batch of images. The system is also perfectly suited to performing complex analyses such as those requiring multiple measurements per image.

The module integrates seamlessly with the cellSens Dimension platform to supply flexible, automated image analysis. The system provides advanced thresholding and spectral unmixing capabilities to facilitate the easy definition of objects of interest, including automatic, manual, manual Hue Saturation Value (HSV) and adaptive methods. This standardises measurement parameters thereby reducing error and variability, while simplifying and speeding up data capture. In all cases, the information acquired can be exported to Microsoft Excel as diagrams, graphs or raw data making the analysis of results straightforward and intuitive.

The Count and Measure Solution makes it easy to create measurement protocols, opening up a range of automated analysis options to users at all levels of expertise. Measurement parameters available include area, aspect ratio, bisector, bounding box, gravity centre, ID, mass centre, intensity values, convexity, diameter, elongation, ferret, extent, next neighbour distance, orientation, perimeter, radius, shape and sphericity. Each of these can be combined and used in conjunction with automatic object detection to perform complex data analysis. This makes it possible to generate comprehensive data sets with minimal user input, all within the same processing run.

The cellSens Dimension software package provides a broad range of advanced features designed to offer maximal control and processing for microscope-based experimental systems.


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