Stem cell classification technique developed at Nottingham University

Share this on social media:

A research team at Nottingham University led by Dr Ioan Notingher has developed a non-invasive Raman microspectroscopy (RMS) technique for identifying live stem cells. The technique phenotypically identifies live cardiomyocyte cells within highly heterogeneous cell populations with greater than 96 per cent sensitivity and specificity. Most techniques currently available for characterising stem cells are invasive and make the cells unusable.

Many medical researchers believe that stem cell therapy will revolutionise the treatment of human disease and may provide treatments for many currently incurable diseases. However, one problem still to be overcome is controlling the excessive proliferation of cells with unwanted phenotypes after transplantation to prevent tissue overgrowth and tumour formation.

The team used an Andor iDus 401A-BRDD cooled, deep-depletion, back-illuminated CCD camera attached to a purpose-built Raman microspectrometer. The system records spectra from individual cells derived from micrometric regions of human embryonic stem cells (hESC). The researchers showed that the technique can be used to discriminate between different cell types.

'We needed the shortest possible acquisition times for the Raman spectra and the Andor iDus 401A camera is ideal for this application, allowing measurements of Raman spectra from selected positions in the cells in only 0.5 seconds,' said Dr Notingher. 'Also, the detectors are optimised for the spectral regions in which we work, 800-900nm, which is vital for avoiding photodamage to the cells.

'Together with Professor Denning, also of the University of Nottingham and a leader in regenerative medicine, we have validated the potential of RMS for allowing the non-invasive phenotypic identification of hESC progeny.'

The iDus camera has a -100°C thermo-electric cooling platform and high sensitivity in the near-infrared (NIR). It is also extremely compact for ease of integration to complex experiments.

Recent News

12 February 2021

Video recorded at 2,800 frames per second has been used to test high-speed trains travelling through the newly opened Ceneri Base Tunnel in Switzerland

29 July 2020

The Perseverance rover contains 19 cameras, including seven scientific instruments. It will analyse the climate and geology of Mars, looking for signs of past life, as well as monitoring the Martian atmosphere

02 July 2020

Norwegian seafood firm, Lerøy, has installed hyperspectral cameras on processing lines to sort fish. The system is able to measure the amount of blood in white fish, which gives a grade of quality

09 June 2020

Hyperspectral imaging is being used in a research programme at hospitals in Maryland and New York to investigate the prognostic value of skin findings associated with Covid-19 infection