Innovative microscope opens up new possibilities for live biological imaging
16 October 2012Tweet
The SiMView light sheet microscope in Philipp Keller's laboratory
Laboratories in Europe and the USA have independently announced in the same issue of Nature Methods the development of new microscopes capable of imaging rapid biological processes in thick samples. The microscopes are ideally suited to the long-term study of live biological specimens.
Philipp Keller designed the microscope used by the group from the Howard Hughes Medical Institute in Virginia, USA, while Lars Hufnagel led the team from the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany. Called SiMView by Keller and Multi-View SPIM (MuVi-SPIM) by Hufnagel, the new four-axis light-sheet microscopes build upon the SPIM (Selective-Plane Illumination Microscopy) technology developed by Keller and Stelzer at EMBL and feature Andor's Neo sCMOS camera at their cores.
Like SPIM, the new microscopes shine a thin sheet of light on the sample, illuminating one layer at a time to obtain an image of the whole sample with minimal light-induced damage. However, SiMView/MuVi-SPIM uses two diagonally-opposed light sources and two cameras to capture four images from different angles, eliminating the need to rotate the sample. This speeds up the imaging and enables the four images to be merged into a single high-quality, three-dimensional image for the first time.
Hufnagel and Keller agree that every aspect of the microscope must be optimised for speed and light sensitivity – optics, mechanics, cameras, and software. Both teams independently chose the 5.5 Megapixel Andor Neo sCMOS camera, which offers a high sensitivity, rapid frame rates of up to 100fps, and vacuum thermoelectric cooling to -40⁰C to minimise dark current and maintain low noise.
‘For global measurements of the dynamic behaviour and structural changes of all cells in a developing organism, data acquisition must occur at speeds that match the timescales of the fastest processes of interest and with minimal time shifts between complementary views,’ said Keller. ‘SiMView/MuVi-SPIM overcomes the limitations of sequential multi-view strategies and enables quantitative systems-level imaging of fast dynamic events in large living specimens under physiological conditions.’
Both teams used the Andor Neo sCMOS cameras to capture a complete view of an embryo of Drosophila melanogaster within a few seconds. Lars Hufnagel focused on the biomechanics of development using one-photon microscopy and recorded the movements of every nucleus in the embryo throughout the first three hours of life when nuclei divide very rapidly as well as producing a 20-hour video showing the fruit fly embryo from approximately 2.5 hours to the stage it walked away from the microscope as a larva. Philipp Keller used both one- and two-photon microscopy, penetrating deeper inside the embryo with multiphoton microscopy to look at subcellular events happening throughout the whole embryo development until eventual hatching.